1. If we looking for detection of estrogen in my sample. the concentration of antigen in sample should vary but the Test line should be same concentration. If I choose a BSA-bound estrogen of 1mg/ml as concentration and use for development of Test line. Is this correct?
2. For sample preparation can we use Phosphate buffer. Are hormones dissolve in Phosphate buffer?
Test line concentrations on LFA are highly variable depending on the assay. I’ve used concentrations as low as 0.125 mg/ml to several mg/ml, so there is no way to answer this question without empirically determining what the optimal test line concentration is. Too high of a testing concentration and you will get false positive results. 1 mg/ml is a good starting point but you should test a range of concentrations.
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