How can I separate 2 sections of a geometry in 3-Matics?

Hi all, I have a reconstructed geometry of a rat liver in a capsule (shown in the attached pic) and it's a unified "one" piece. I need to cut out the capsule and separate the capsule and the...

05 April 2020 5,657 1 View

How to measure nerve compound action potential ex-vivo in a "reproducible" way?

Hi all, I'm trying to measure the compound action potential (CAP) on an isolated porcine phrenic nerve which is stimulated on the proximal end but every time I remove the nerve from the electrode...

18 February 2020 4,831 2 View

What is the best approach to determine the compression rate for scientific text summarization? Is there any formula for it?

I want to determine the compression rate of scientific articles which I'm going to summarize automatically for my current project. Since, the length of articles varies and I also want to summarize...

14 November 2019 8,009 3 View

How long can I keep dissected rat sciatic nerve in oxygenated Kreb's solution for my in-vitro experiment? is it the right solution for Sciatic nerve?

Hi all, I was wondering if I should use oxygenated Kreb's solution to store my dissected rat's sciatic nerve before my experiments and also how long can they survive in that? (by survive I mean...

20 October 2019 8,938 2 View

Why using the same protocol I cannot see any spots in my 2d gel results sometimes?

I am trying to do 2d gel electrophoresis for the whole protein of cell lysate. and using aceton precipitation I clean up my lysate. I have Urea, CHAPS and IPG buffer3-10 in my rehydration buffer....

16 June 2019 881 11 View

How hydroxyapatite affects polymers characteristics?

polymer-ceramic compositions properties

05 March 2018 9,250 4 View

How the red blood cells (RBCs) spread collectively in a microchannel?

As we know microfluidics provide opportunities to stimulate, and diagnose blood cells.the important aspect in the design of microfluidic devices is to Control the concentration of cells at every...

25 February 2018 9,637 3 View

What are the bio mechanical consequences of running with deep core muscle weakness?

Since deep core muscles are mostly neglected i wanted to know the results and how this weakness affect the shear modulus in spine.

24 February 2018 8,345 2 View

Can anybody suggest me a way of Graphene Quantum Dots synthesis that has Near IR absorption?

I am trying to synthesize GQDs by a practical way but the prevalent methods contribute to blue or green absorption. I need a process to have Near IR.

22 October 2015 2,520 1 View

How can I estimate the cell confluency percentage in cell culture flasks?

15 July 2015 4,304 10 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View