Hi all,
I'm trying to measure the compound action potential (CAP) on an isolated porcine phrenic nerve which is stimulated on the proximal end but every time I remove the nerve from the electrode ladder (shown in the attached file "1.png") and put the nerve back on it again right after that, I don't get the same action potential because the position of the nerve relative to the electrodes have changed a bit! how can I measure the action potential in a more reproducible way? is there a better available setup?
P.S: I need to remove the nerve from the ladder after every measurement and I cannot keep it on the recording system all the time.
Hi Pegah Ranjbartehrani ,
There is a method called "air-gap" that has been well-established and employed in several studies mainly to record CAP from sciatic nerves in swine and mostly in frogs. Though I am not sure this would work, it seems that you would get a stable positioning of your nerve every time you would record. The setup is different and you may not need to remove the nerve every time from the plate.
The paper have references and contacts to seek more details on the methodology. I think it would worth it to have a look:
https://www.sciencedirect.com/science/article/abs/pii/S0378874115302683?via%3Dihub
There is an older paper that can also be useful:
Article Blocking effects of hypoacomte and aconitine on nerve action...
Use a two dimensional setup. Puting the nerve onto a saline solution wet filter paper and jsing a monopolar electrode. This was used by Lorente de No in the 30s last siecle. I have written about this in
Int J Biomed Comput, 26 (1990) 211-220. 211
TEACHING FIELD POTENTIALS: A MICROCOMPUTER SIMULATION OF THE NERVE ACTION POTENTIAL IN A BIDIMENSIONAL CONDUCTOR
Enrique Soto and Rosario Vega.
is full text avail here in Research Gate
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