How to measure nerve compound action potential ex-vivo in a "reproducible" way?

Hi all, I'm trying to measure the compound action potential (CAP) on an isolated porcine phrenic nerve which is stimulated on the proximal end but every time I remove the nerve from the electrode...

18 February 2020 4,831 2 View

What is the best approach to determine the compression rate for scientific text summarization? Is there any formula for it?

I want to determine the compression rate of scientific articles which I'm going to summarize automatically for my current project. Since, the length of articles varies and I also want to summarize...

14 November 2019 8,009 3 View

How long can I keep dissected rat sciatic nerve in oxygenated Kreb's solution for my in-vitro experiment? is it the right solution for Sciatic nerve?

Hi all, I was wondering if I should use oxygenated Kreb's solution to store my dissected rat's sciatic nerve before my experiments and also how long can they survive in that? (by survive I mean...

20 October 2019 8,938 2 View

Why using the same protocol I cannot see any spots in my 2d gel results sometimes?

I am trying to do 2d gel electrophoresis for the whole protein of cell lysate. and using aceton precipitation I clean up my lysate. I have Urea, CHAPS and IPG buffer3-10 in my rehydration buffer....

16 June 2019 881 11 View

How hydroxyapatite affects polymers characteristics?

polymer-ceramic compositions properties

05 March 2018 9,250 4 View

How the red blood cells (RBCs) spread collectively in a microchannel?

As we know microfluidics provide opportunities to stimulate, and diagnose blood cells.the important aspect in the design of microfluidic devices is to Control the concentration of cells at every...

25 February 2018 9,637 3 View

What are the bio mechanical consequences of running with deep core muscle weakness?

Since deep core muscles are mostly neglected i wanted to know the results and how this weakness affect the shear modulus in spine.

24 February 2018 8,345 2 View

What is high background fluorescence in ISH related to?

11 April 2016 7,041 1 View

Can anybody suggest me a way of Graphene Quantum Dots synthesis that has Near IR absorption?

I am trying to synthesize GQDs by a practical way but the prevalent methods contribute to blue or green absorption. I need a process to have Near IR.

22 October 2015 2,520 1 View

How can I estimate the cell confluency percentage in cell culture flasks?

15 July 2015 4,304 10 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

Hi all, My research questionnaire has 3 sections each having a mix of yes/no and multiple choice questions. How can I test its reliability ?

The sections are about awareness, practice, and perception regarding waste management. Thanks

20 February 2021 5,722 3 View

Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View