Leonhard Möckl

3 Questions 21 Answers 0 Followers

Questions related from Leonhard Möckl

Short-term storage of E. coli: +4, -20, -80°C?

Hi everyone, for a set of experiments I would like to store E. coli for four to five days. I did some literature recherche on short term storage of bacteria found a lot of different ways to do...

02 June 2016 7,870 9 View

How to reduce unspecific binding in an intracellular click-reaction?

Good morning, we are performing an intracellular click-reaction using an azide coupeled to an intracellular protein and a TAMRA alkyne (after cultivation, the cells are fixed, permeabilized,...

23 June 2015 4,802 6 View

Is polycarbonate attacked by chloroform?

Hi everyone, I want to extract lipids via the Folch-method. For this, I have to incubate cells with a chloroform-methanol-mixture. After this, I need to centrifuge the suspension. We have a nice...

09 April 2015 9,043 5 View