I am performing mass spec data analysis on proteome discoverer. There is a percolator node in the workflow. I wanted to know what does it do?
I have protein samples which I need to identify. I loaded it on SDS PAGE. Now each band may contain two or more proteins. Can I estimate relative abundance of those proteins through IBAQ? If yes,...
05 June 2019 6,131 3 View
My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.
04 May 2018 1,828 1 View
I have already cut the gel pieces and would like to perform in gel digestion in future. So I had freeze them in 1% acetic acid. Can I still process those samples?
31 December 2017 3,059 1 View
My project involves analyzing proteome of my sample. I have more than 100 samples for analysis. Would proteomic analysis in R help me? Is it better than other softwares?
31 December 2017 6,581 8 View
I am working on snake venom proteins and wanted to resolve it on SDS PAGE. I have attached image of my gel. I am getting a smear. Is there any way to resolve it better?
04 May 2016 9,119 5 View
I was reading an article where they have mentioned alleles of polymorphic markers. they are talking about U2 tandem arrays.
02 March 2016 8,035 2 View
I know that it gets sandwiched between glycine and Cl ions But how does it get compress literally?
01 February 2016 7,311 2 View
I was reading SDS PAGE and methodology it was mentioned, I searched it online but i couldn't find the answer.
01 February 2016 506 5 View
I wanted to understand gene set enrichment analysis. For that I need some practical examples of gene-lists and gene-set used in this technique
09 October 2015 7,558 4 View
I have to quantify expression of HSP proteins in frog. So what could be best possible technique to do that?
04 May 2015 5,343 3 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View