What is the best method of lysing Osteoblast sarcoma cells (SaOS-2)?
Does fast heating make a difference?
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
06 August 2024 2,508 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...
03 August 2024 2,546 2 View
I was hoping anyone could provide any insight into using ATCC's "RD-ES" and "A-673" cell lines. How many passages do you do before you notice any senescence or genetic abnormalities
20 May 2024 596 4 View
symptoms and clinical examination
22 March 2024 5,286 2 View
I am planning to create xenografts of solid tumor cells (sarcoma) in NOD/SCID mice. Our lab has previously established such tumors in these mice but with a certain cell type we have not been able...
17 March 2024 2,946 3 View
Recently, we have been encountering challenges in our laboratory in achieving satisfactory mineralization of MC3T3-E1 cell line. We would like to determine the optimal concentration of Ascorbic...
13 March 2024 7,500 1 View
Dear colleagues, I tried to transduce mouse osteoblastic cells with retrovirus many times but I failed, do you have any recommendations? Thank you in advance.
25 November 2023 1,643 0 View
We are doing primary osteoblast culture from mouse calvaria, but we always get those very big cells as shown in the red cycle of the picture, which takes a lot of areas and blocks the real...
13 November 2023 8,879 1 View
Hi, im looking for mice osteoblast cell line ( not MC3T3) for in vitro work. any suggestions?
31 October 2023 8,077 2 View
Need to make osteoblasts by transdifferentiating fibroblasts but can only use immortalized human fibroblasts (not primary cells); will this even be possible or would I absolutely need to reverse...
15 October 2023 7,085 0 View
Hi, I am having trouble marking the calcium deposit of osteoblasts from the MC3T3-E1 Sub 14 lineage. After staining with alizarin red, it is not possible to observe the presence of mineral...
21 August 2023 9,529 3 View
Our lab has been trying to perform knockouts of genes involved in the WNT pathway in osteosarcoma and Ewing sarcoma cell lines (HOS, SAOS2, U2OS, MHH, A4573). When we are able to successfully...
01 August 2023 9,002 3 View