Joel Turner

9 Questions 6 Answers 0 Followers

Questions related from Joel Turner

Can you remove samples from TEM grids?

Is it possible to remove nanometre resin sections containing cells from TEM grids without removing the existing coatings e.g Formvar/carbon coats Thanks in advance!

21 November 2022 2,753 7 View

Can monolayer cells that have been fixed in aldehydes been removed from a plate using trypsin?

Cells are fixed in PFA for TEM in cell culture flasks. Is there a way to remove fixed cells without scraping?

04 May 2022 1,789 0 View

Is there a coverslip for cell culture that is conductive?

I would like to be able to mill cells with FIB and then perform slice and view with an SEM. I hear milling is difficult without placing cells conductive surface even after coating cells with...

01 September 2021 7,312 4 View

How do I stop TEM samples being destroyed under electron beam?

The samples that I make for TEM contain cells. I need them to be 70-90nm (no higher) to be able to see them clearly and do EDX as well. However, They begin to rip and flex under the beam even at...

20 August 2019 2,551 1 View

What is the best technique for elemental mapping of biological samples in STEM?

Existing techniques of elemental mapping in STEM include: EDX, EELS, XRF, EFTEM, HAADF. Which is the best for mapping elements in biological samples??

16 April 2019 5,941 6 View

What is better for lysing cells prior to Total DNA assays: Slow or Fast freezing?

What is the best method of lysing Osteoblast sarcoma cells (SaOS-2)? Does fast heating make a difference?

12 November 2018 8,351 0 View

Which are better multi-channel or single hollow tube conduits for peripheral nerve regeneration?

What are the pros and cons of each approach?

06 March 2018 9,804 1 View

Which characterisation techniques should I use to find the electronic structure, crystal structure and surface structure of titanium dioxide?

I need to conduct some research on TiO2! I could use some help!

31 January 2018 2,403 6 View

Why are sol-gels better than hydrogels for cell cultures?

What are the benefits?

30 October 2017 5,566 3 View