the first questions to make is why get a patent? Is there a commercial application for the work? Where is the commercial venture going to succeed in terms of geographical region?
After that the next question to answer is on whether the work qualifies for a patent.This varies from jurisdiction to jurisdiction so where is the commercial venture going to succeed answer is imperative.
For example in the US jurisdiction the criteria that it needs to be an invention and not a natural process is essential. Has this invention been used, described anywhere in the world? Have you searched exhaustively to determine this originality?
After you answer these questions positively towards establishing that you have a unique invention then you can proceed with considering a patent. I strongly suggest that you contact an IP professional at this stage to get further advise.
Note: I noticed that you are a research associate at a Sant Gadge Baba Amravati University. Please make sure that you check with the University's technology transfer office on proper procedure if conducted during the normal course of your work.
Follow Arturo's advice. Also note that you cannot get a patent if the invention has been published or demonstrated anywhere in the world before the date you apply for it. So ensure no publication or talks about about the invention before you apply. Having applied for the patent in your country, you then have 12 months to apply for patents in other countries. It's essential you talk to your University's technology transfer or intellectual property people to get advice. Of course, also remember that applying for a patent costs money!
I agree with the above responses. If your research is research within a University you most probably have a duty to report this to the Tech Transfer Office and they will guide you through the rest of the process. If your research is not performed within a University, you first should consider the commercial importance of the invention and whether or not you would be able to find investors for providing the money for developing (and patenting) your invention. Or, determine whether there would be people who could pay your money to use the invention (i.e. be your licensee). Of course, in all of these activities you should prevent publication of your invention, which means that any discussions with other people/companies shoudl be done under confidentiality conditions.