What is best method forTransfection of MCC line?

More Kashif Rasheed's questions See All

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

How the dropped packet is being transmitted in RPL DODAG?

I am implementing an enhanced objective function for RPL. Control messages and data packets of UDP applications are being transmitted. Now the question is that if any data packet which is being...

02 June 2024 3,418 2 View

What is the best method for diagnosing biofilm formation on liquefaction tubes used in poultry farming fields?

biofilm

31 May 2024 1,969 4 View

Why does calcium deficiency occur in humans despite eating calcium-rich foods?

About deficiency of calcium

24 May 2024 9,263 2 View

How can I effectively utilize red phosphorus to synthesize Cu3P given its solubility issues in water and organic solvents?

I am trying to synthesize Cu3P, but I am encountering difficulties with the solubility of red phosphorus in water and various organic solvents. What should I do in this situation? Could anyone...

24 May 2024 7,753 2 View

How to calculate the activation energy for grain and grain boundaries?

how to calculate the activation energy for grain and grain boundaries?

18 May 2024 5,413 0 View

Can you help in Linkage Analysis Tool?

Can anyone assist with running the Linkage Analysis Tool 'Easylinkage' or any alternative tool for conducting linkage analysis and calculating LOD scores?

06 April 2024 7,273 2 View

How to control the CO radical in the photocatalytic reaction?

I am doing a reaction in which terminal alkene was broken down into ketone, aldehyde, and another by product aldehyde (formaldehyde). The problem is that this formaldehyde reacts with another...

31 March 2024 7,080 2 View

How to design Control system for the RO process?

I'm currently tasked with designing the control system for a reverse osmosis (RO) feasibility project. I'm looking for guidance on how to approach this design process and what software options...

27 March 2024 9,751 3 View

Can we measure quantum yield of carbon quantum dots using Rhodamine B as standard solution ?

I want to measure fluorescence quantum yield of carbon quantum dots, can we use Rhodamine B as a standard solution and also explain the whole procedure of measuring quantum yield.

18 March 2024 1,252 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Preeti Kapoor

Have you tried nucleofection like the 4D nucleofector system from Lonza

David Schrama

Dear Kashif,

We also had only low transfection efficancy with lipofectamine. Therefore, we now do everything with lentiviral transduction. That works fine. So if you have the possibility to work with lentivirus I would suggest giving that a try.