I want to measure fluorescence quantum yield of carbon quantum dots, can we use Rhodamine B as a standard solution and also explain the whole procedure of measuring quantum yield.
Rhodamine B is the most commonly used standard for QY. Whether the standard solution is appropriate for your sample depends on PL spectra and spectrometer calibration. Ideally, the standard should match the spectrum of the sample of interest as closely as possible to minimize spectrometer calibration errors. On the other hand, to minimize QY variations due to handling procedures you can cross-calibrate with another standard solution.
Check the following reference for a detailed discussion about QY standards and the attached file for a practical guide on relative QY measurements using a standard solution.
Article Standards for photoluminescence quantum yield measurements i...
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