What is best for cell cycle analysis, DAPI or PI?

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Alison Howarth

It may be that you are getting different results as PI interacts with both DNA and RNA, so I would suggest that you use an RNase before adding PI to make sure it is specific to DNA.

Shin-Ichiro Hiraga

I prefer DAPI, because;

DAPI is (almost) specific to DNA, and does not required RNase treatment

DAPI's fluorescence is less likely to interfere with other channels (=colours).