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Questions related from Enrique Espinosa
When I gate singlets, lymphocytes, CD3 and then CD8 in my cytometry analysis, I see that CD8+ lymphocytes are divided in two. One population is very bright, and the other is less bright although...
28 January 2019 6,876 4 View
I have discordant findings using DAPI and propidium iodide. Should I trust any over the other? I study human memory CD4 T cells.
06 September 2018 9,228 2 View
I need to measure intracellular NAD (total and NAD+) in human T cells. I see there are several systems (colorimetric and ELISA), and I need a kit that is reliable. I don't want to use HPLC for...
09 August 2018 1,666 1 View
Do Jurkat and other CD4 T cell lines express CD38? Ideally I would like to have a CD38neg and a CD38pos line. I got lost in the ATCC web page trying to find subclones of the main cell lines. I...
18 September 2017 5,558 2 View
As many other researchers, I'm having a hard time measuring plasma lipopolysaccharide levels by LAL (limulus amoebocyte assay). I will try ELISA. I have found two manufacturers: Aviva Biosystems...
26 June 2017 8,226 1 View
We are going to clone a protein in a pCMV-Myc plasmid. Looking for fluorochrome-conjugated antibodies to track the efficiency of transfection, I found both cMyc and Myc antibodies. I don't know...
13 September 2016 466 6 View
LPS is obtained from different bacteria, and their activities may differ. Additionally the degree of glycosilation also influences LPS activity. Which origin or brand or kind of LPS yields a clear...
19 May 2016 6,478 5 View
I have access to peripheral blood mononuclear cells that have been cryoprepreserved and stored in liquid nitrogen for 10 years. They belong to an interesting cohort. Cells that have been...
17 December 2015 5,813 12 View
We need to determine NAD (total: NAD + NADH) concentrations in plasma, supernatants from cell cultures, and in cell lysates. We recently had a very bad experience with a kit from another...
22 June 2015 7,327 1 View
I'm running an experiment in whole PBMCs, from which I need to identify CD4 and CD8 T cells, as well as monocytes by flow cytometry. I am familiar with the markers and gating strategies required...
19 May 2015 7,070 8 View
We have plasma samples kept in liquid nitrogen for seven years. Can they still be used to measure cytokines? We are very interested in measuring IL-1, IL-18, and any other related cytokine (IL-6,...
17 April 2015 7,082 4 View
We measured the induction of a series of genes in purified subsets of T cells cells, by polyclonal stimulation through the TCR. Since the conditions varied slightly between experiments, we...
04 November 2014 496 4 View
There are many markers of endothelial activation and damage (sVCAM-1, von Wilebrand factor, D-dimer, etc. etc. etc.), and I need to decide for one (ideally) or two to measure in human blood that...
14 October 2014 725 15 View
Is there a signaling pathway or a particular signal induced by arrest between S phase and mitosis (cells don't divide after synthesizing new DNA) that initiates cell death? Is this death...
05 September 2014 501 6 View
We are analyzing memory CD4 T cell responses (cytokines, co-stimulatory molecules and chemokine receptors) before and after TCR-mediated stimulation. GAPDH gave nice parallel efficiencies with the...
25 September 2013 4,317 12 View
I am going to use Click-iT Edu reagent and protocol to detect cell proliferation by flow cytometry, on cells staining for extracellular and intracellular proteins. The reagents are compatible with...
08 March 2013 980 12 View
I need to sort cells (PBMCs mainly) from patients with untreated HIV, active tuberculosis and pandemic H1N1. So I need the cytometer to fit into a Class II Type A2 Biological Safety Cabinet or...
21 October 2012 3,461 3 View
