What is ASS assay, ADI assay and what is Enzymatic Assay ?
ASS and ADI are enzymes which have to do with metabolic pathways involving citrulline.
ASS is argininosuccinate synthase. By means of ATP, this enzyme catalyzes the formation of argininosuccinate from aspartate and citrulline.
ADI is arginine deaminase which catalyzes the formation of citrulline from arginine. Additionally ammonia is formed by this reaction.
I did an experiment couple of days ago, the aim of the experiment I did was "differentiate U937 monocytic cells into macrophages usingPhorbol 12-myristate 13-acetate (PMA) and then quantify TNF-α...
11 December 2019 7,878 5 View
Hello everyone I am writing a lab report about TNF-a and LPS. I tried to find journals about this but I can't find anything online and I am really confused why does LPS lead the cells to make TNFa?
11 December 2019 5,106 3 View
Why it is important to assay L-citrulline and L-arginine?
01 January 1970 5,898 3 View
Hello Everyone, I am trying to find how many samples can you go through with Enzymatic Assay method ? (assaying l-citrulline and l-arginine) I am using this research article to find the...
01 January 1970 7,655 3 View
How can I write about trends for the values I obtained ?
01 January 1970 4,123 4 View
Dear All, What is the principle of enzymatic assays? What is ADI assay and ASS assay ?
01 January 1970 1,320 2 View
Dear All, I would like to assay L-Citrulline and L-Arginine, however, I am not sure if I should use RP-HPLC or Enzymatic Assays. How many samples can I get through in a day with these techniques...
01 January 1970 5,682 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View