What is ASS assay and ADI assay?

What is ASS assay, ADI assay and what is Enzymatic Assay ?

31 December 2019 5,440 1 View

TNF-alpha experiment, how to explain the results?

11 December 2019 7,630 5 View

Why does LPS lead the cells to make TNFa?

Hello everyone I am writing a lab report about TNF-a and LPS. I tried to find journals about this but I can't find anything online and I am really confused why does LPS lead the cells to make TNFa?

11 December 2019 4,858 3 View

How many samples can you go through with Enzymatic Assay method ?

Hello Everyone, I am trying to find how many samples can you go through with Enzymatic Assay method ? (assaying l-citrulline and l-arginine) I am using this research article to find the...

01 January 1970 7,568 3 View

Why it is important to assay L-citrulline and L-arginine?

Why it is important to assay L-citrulline and L-arginine?

01 January 1970 5,827 3 View

How can I write about trends in values obtained without actually doing any graph?

How can I write about trends for the values I obtained ?

01 January 1970 4,036 4 View

Assaying L-Citrulline and L-Arginine

Dear All, I would like to assay L-Citrulline and L-Arginine, however, I am not sure if I should use RP-HPLC or Enzymatic Assays. How many samples can I get through in a day with these techniques...

01 January 1970 5,606 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View