Hi,
What is the most appropriate lysis buffer for measuring β-hexosaminidase? I tried a 1% triton-x-100 in Tris-HCl solution and refrigerated sample for 20 minutes but that did not seem to work.
Thanks!
Hi everyone. Need a help here. I'm currently doing a source rock characterization for my research. However, I'm a bit confuse on how to calculate original TOC and HI from several complicated...
22 February 2024 3,464 0 View
What is the process of conducting reliability test and validity test for semi-structured interviews?
06 July 2022 7,600 3 View
We have proceeded in a complete acid degradation process of lercanidipine drug and we separated the degradation products by HPLC to obtain 5 degradation peaks, then for further identification and...
18 October 2021 666 4 View
Hello, I am working with mast cells suspension cells (i.e. BMMCs and HMC), and I was wondering if anyone has any experience working with these cells. Specifically performing a degranulation assay...
07 March 2021 813 1 View
Hello, We are currently working on an inflammatory model using a mouse air pouch and we are having some trouble with evaluating gene expression using this tissue. We have been using the Qiagen...
22 November 2020 1,174 1 View
1) How can we extract the magnitude and the phase of the transmission and the reflection of the antenna structure. 2)If an Antenna radiates in backward Direction, What can be be the reasons...
17 September 2020 6,740 3 View
Need to change Hossam Kandil picture and affiliation Hossam Kandil Professor of Oral maxillofacial radiology, Cairo University Not Cardiology This is wrong Kindly help me correct the...
02 August 2020 5,309 5 View
Hello,I am trying to check whether there is a positive or negative relationship between two variables. Agreeableness (which is made up of 9 questions) and Emotional intelligence (which is made up...
11 August 2019 623 38 View
Can anyone give me the step by step procedure to find the value of magnetic saturation using the law of approach to saturation for BaFe12O19 sample? Thanks!!
06 March 2019 2,717 1 View
Consider the following: The role of education to a. to develop young peoples’ sense of belonging in multicultural Britain through developing interfaith dialogue. b. to enhance young peoples’...
22 June 2018 3,372 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Require for Rietveld refinement in XRD.
08 August 2024 3,081 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View