I am looking for the most simple but still reliable method to determine the hemoglobin amount in a liposuction sample. There are some special colorimetric kits made for hemoglobin assay, but they are quite expensive and there is no proof that they could be suitable for usage in my case. Maybe somebody knows a reagent which is included in such a kit? Or maybe somebody tried to determine hemoglobin in lipid-enriched samples and could share your experience?
Interesting question. I am afraid you are going to need a calorimetric assay for this purpose because of the nature of the sample that is likely to contain lots of fat and fatty particles. It will clog up any automated analysers, If you have no access to colorimeter and you are not too bothered about the precision, you could use a very old technique that is really semiquantitative at best- Tallqvist method using a blotting paper and a colour chart. Good luck
Siva
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