Dear all,
We need to construct a plasmid that has different than neomycin, puromycin or blasticidin resistance (as we are using them in our other vectors).
Can anyone share a sequence of hygromycin resistance gene or any other antibiotic resistance gene (except mentioned above) that can be used for selection in mammalian cells.
Dear Malgorzata
you can extrapolate it from the sequence of pcdna5/frt https://www.thermofisher.com/order/catalog/product/V652020
which contain the hygromocin
https://tools.thermofisher.com/content/sfs/vectors/pcdna5frtto_seq.txt
in thermo website you can find also other vectors carryng other resistances as zeocin ( eg pftr/laczeo)
good luck
Manuele
ATGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGA TGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCG GGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTC CCGATTCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCAC AGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCAT GGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGT CAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGA TGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCC CGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACA GCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGA GGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATC GCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAAT TTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGC GTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGG AAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAG
may be depending on THE SOURCE OF GENE THAT ISOLATE FROM MICROBE OR PLANT OR ANIMAL CELL AND PCR TECHNIQUE