Dear Fellow Researchers,
Can you recommend a suitable antibody recognizing human CD8 in paraffin sections of cancer samples? We want to combine it with other markers and do an analysis by immunofluorescence microscopy.
Best,
Malgorzata
I'm afraid most of these anti-CD8 reviews are for FC, but still might be worth a quick look:
https://pabmabs.com/?cat=1786
Does anybody know how to convert IU/ml in case of anti-TSHR antibodies (Graves disease)? I would like to know how much anti-TSHR Ab I have in a sample
02 February 2024 1,625 3 View
I expected age between present up to 100 years. I heard U-Th is useless in this case. If you have any idea let me know, please.
27 January 2024 8,273 7 View
What are the rules when choosing antibodies
04 September 2023 4,832 4 View
I am looking for examples of practical use of randomly distributed natural fibers to improving the soil, e.g. under the foundation or as the top layer of a dirt road, or other. In the literature,...
22 June 2023 340 0 View
Hello everyone, I would like to ask about thermal decomposition of polystyrene using TG-QMS system - thermogravimetry coupled to quadrupole mass spectrometer. During thermal degradation of pure...
14 February 2023 8,556 3 View
Dear Fellow Researchers, We have analyzed the effect of various parameters on bladder cancer recurrence. Among included parameters, we had various continuous parameters. In the literature,...
20 August 2022 6,514 4 View
I am interested in the minerals particles suspended in seawater. What are the minerals? Carbonates, silicates, what more? Which clays are the most common? Could someone provide articles related to...
19 August 2021 6,542 6 View
I got CLS4621 and Corning Matrigel 356234
23 May 2021 974 0 View
After fitting Mossbauer spectra for Fe(II)-silicate heated at air to 700degC, I observed uncommon, very high quadrupole splitting parameters for Fe3+, I suspect in very disordered environment (?)....
03 May 2021 5,143 5 View
Hi All! I am using ITC to study binding of lectin Concanavalin A to glycopolymer decorated with around 40 saccharide moieties (MW ~ 16 000 Da), which is multivalent-type ligand. Binding of...
12 April 2021 3,571 5 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?
04 August 2024 1,537 1 View