I have a sample soluble in water. To carry out the Thin Layer Chromatography (TLC), which organic volatile solvent is recommended? It is also soluble in DMSO, however DMSO is not a volatile solvent. It is also not soluble in Ether, DCM, EA, EtOH, Hexane, CHCl3, etc. For example, the reaction is A + B --> C, here A & C are soluble in Ether, DCM, EA, EtOH, Hexane, CHCl3 but B is not, whereas B is soluble in water but not in Ether, DCM, EA, EtOH, Hexane, CHCl3. The eluent I am using for the TLC experiment is Hexane:EA. Two maerials (A & C) are responding in the Hexane:EA = 2:8 to 5:5, however B is not. In fact, B is not responding in Hexane:EA = 2:8 to 9:1. Kindly suggest. Thank you in advance.