Can it happen with continuous passage of pure cell culture ? Can the cell change its features?
1) More than one cell type in a given culture, aka contamination. 2) maybe with inconsistent technique, 3) Yes, but less likely.
Blake Warner
I guessed another kind of cell not change in the features .
Divergence can happen with subtle changes in media preparation or types of stimulants or growth factors that you might put in the media will differentiate more do you differentiate your cells also over time your cells of their primary cells will also differentiate or change me to become immortalized and obviously there much different than their primary their primary cells especially if they were normal, things that you should be aware
I think it is much more likely that either a there were more than one cell type to start out with or be you got contamination from one of the other cell lines that you're working with I think it's less likely for you to start out with a homogeneous outline and then it up with a heterogeneous outline given that was a pure culture
I agree with blake. it is far too common to have one cell line contaminating another one, especially when they share the same medium. some time ago, a study on several cell lines showed that they were, actually, all hela cells!
- Badges
- Science topic
- Similar topics
- Methodology
- Laboratory
- Cellular Biology Techniques