Blake M Warner

3 Questions 25 Answers 0 Followers

Questions related from Blake M Warner

Has anyone isolated high quality RNA from RIPA lysed cultured mammalian cells?

Hello, I would like to isolate RNA from RIPA lysed cells treated in vitro. Has anyone done this? Can I just use a kit (Qiagen RNeasy, or other) to perform this or are there additional steps...

09 September 2014 3,991 10 View

Have you quantified histopathologic premalignant lesions in the rat 4NQO oral squamous cell carcinoma model?

1) Do you count discrete lesions (low-grade, high-grade, CIS, SCC) per section? Per tongue? Anterior, middle third? Or do you simply assign the highest grade to the tongue as a whole? I have done...

02 February 2014 1,854 1 View

Anyone have a robust transfection protocol for oral keratinocytes?

I tried using the SA Biosciences Cignal Finder Pathway Reporter Array to examine the pathways activated by a putative chemotherapeutic agent (l). I followed the manufacturer's directions to the...

04 April 2013 9,707 2 View