What does mature protein mean? What are the characteristics and description for a protein to be called mature?
I transformed a purified 11Kb plasmid (via plasmid mini prep) from an E.coli recombinant strain into the target host via electroporation. I got colonies but there was no DNA band during colony...
09 October 2017 3,803 12 View
Problem: My WT has the highest fluorescence data than strains with empty vector and GFP-containing. Protocol: 1. I inoculated all my GFP - M.organophilum strains into a Choi medium - a medium...
09 October 2017 6,131 5 View
If I have multiple points where it is linear, should I use all the points that are linear to account for the calculation of specific growth rate?Also, how linear should the line be? what are the...
06 July 2017 767 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
23 July 2024 4,074 2 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
24 June 2024 5,044 1 View
Hi everyone, I was wondering how to get the correlation between one dependent variable and independent variable, Pearson correlation is not an option as there is a follow up visit for each...
24 June 2024 8,104 6 View