What does mature protein mean? What are the characteristics and description for a protein to be called mature?
I transformed a purified 11Kb plasmid (via plasmid mini prep) from an E.coli recombinant strain into the target host via electroporation. I got colonies but there was no DNA band during colony...
09 October 2017 3,696 12 View
Problem: My WT has the highest fluorescence data than strains with empty vector and GFP-containing. Protocol: 1. I inoculated all my GFP - M.organophilum strains into a Choi medium - a medium...
09 October 2017 6,016 5 View
If I have multiple points where it is linear, should I use all the points that are linear to account for the calculation of specific growth rate?Also, how linear should the line be? what are the...
06 July 2017 681 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...
17 February 2021 8,986 2 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...
12 February 2021 294 4 View
Hi I've been looking for a stat package for my sensory evaluation data (progressive profiled which is a multi-timepoint version of descriptive analysis). Is there any available one to...
08 February 2021 9,170 3 View
I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...
07 February 2021 3,880 9 View
I used descriptive statistics, the Mann-Whitney tests (male/female responses), The Kruskal Walis test (3 different job title categories), and was considering which other test I could use. I also...
05 February 2021 7,238 5 View
Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...
04 February 2021 4,482 4 View