Rey Sanjorjo

4 Questions 8 Answers 0 Followers

Questions related from Rey Sanjorjo

What instances that cause false positive colonies during transformation using a purified plasmid?

I transformed a purified 11Kb plasmid (via plasmid mini prep) from an E.coli recombinant strain into the target host via electroporation. I got colonies but there was no DNA band during colony...

10 October 2017 3,763 12 View

What are possible sources of false positive fluorescent readings in EGFP assay?

Problem: My WT has the highest fluorescence data than strains with empty vector and GFP-containing. Protocol: 1. I inoculated all my GFP - M.organophilum strains into a Choi medium - a medium...

10 October 2017 6,094 5 View

When plotting the semi-log graph for finding the specific growth rate what are the considerations?

If I have multiple points where it is linear, should I use all the points that are linear to account for the calculation of specific growth rate?Also, how linear should the line be? what are the...

07 July 2017 739 4 View

What is a mature protein?

What does mature protein mean? What are the characteristics and description for a protein to be called mature?

09 September 2016 5,442 0 View