Hi,
I am asking a question regarding the inbreeding of western Tragopan by using GBS, but we have only 12 samples from a single valley, it may be enough for analysis, and which tool do we have to use for analysis? Please share your valuable comments and relevant pieces of stuff.
thanks
Hi,
12 individuals from a single population seems quite a small sample. You will be able to rank individuals by degree of inbreeding, but the values will be relative to the population you have sampled. Runs of homozygosity analysis might be useful, but you would need a good reference genome and high sequencing depth for each observation.
See our project https://www.researchgate.net/project/Genotyping-for-production-and-security-in-a-biological-economy-an-MBIE-project for some methods and examples of applications of GBS.
I am following your project, this is conservation concern species that's why we are unable to collect the samples and rare as well. so we will try to best analysis by these samples,\.
thanks
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