We are interested in studying the effects of iron loading in the brain on the structure of myelin in a mouse model using transmission EM (we have no EM background). Colleagues have informed us that there are various fixation methods optimised for different structures in the brain - i.e. that a fixation method that works for cerebral grey matter may not necessarily be the best for myelin. I was wondering if anyone could point me to a good EM resource for fixation, or had any tips or tricks that work for fixing myelin for TEM?

Thanks!

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