Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Has anybody seen research on Generative Adversarial Networks not image / video / autoencoder based?

Searching for Generative Adversarial Networks applications.

09 October 2020 2,316 2 View

Can the crystallinity of starch or other materials be calculated from the birefringent area?

Hi I would like to know if is it possible to obtain the crystallinity of a material such as starch from the ratio of the birefringent area to total area using the images obtained from light and...

30 March 2020 952 5 View

Why gap opening is fixed in instruments when using cone-plate geometry?

Hello I work with starch gels and I am performing experiments using cone and plate geometry with 1mm of gap because that height allows me to handle the samples better. I still can not understand...

28 March 2019 1,532 8 View

Does Golgi-cox stanning work in tissues storaged in sucrose 30% for 1 month before the stainning?

11 December 2018 5,386 1 View

If I want to do PCR of Cryptosporidium and Giardia to test, is it better to use a nested one?

05 June 2017 2,654 7 View

Can anybody provide some possible methods to dispose heavy metal contaminated soil, specifically Cd contaminated soil for Cocoa Cultivation?

Can anybody provide some possible methods to dispose heavy metal contaminated soil, specifically Cd contaminated soil for Cocoa Cultivation? superficial soil layers (30cm) large land...

02 March 2017 5,722 9 View

Where is the problem with bad results on miRNA RT-qPCR?

In our lab we are performing an miRNA isolation from rat liver with Trizol (including the extra step for helping in the recovery of miRNAs) and then we perform the RT reaction with Taqman microRNA...

11 April 2016 3,121 8 View

Have you used 'text mining' to tackle ghostwriting?

07 August 2015 4,833 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View