Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

How to set up directed evolution to select for membrane recruitment?

Hello, I have a protein which binds the plasma membrane of cells. I'd like to do directed evolution to make its membrane binding affinity stronger, but I'm having trouble with figuring out the...

06 October 2020 2,220 1 View

Can the crystallinity of starch or other materials be calculated from the birefringent area?

Hi I would like to know if is it possible to obtain the crystallinity of a material such as starch from the ratio of the birefringent area to total area using the images obtained from light and...

30 March 2020 952 5 View

Is it advisable to include indicators with different scales in a SmartPLS measurement model?

I have only ever used similarly scaled items in my PLS models. Logically, I can see that would be possible to rescale items with more categories to smaller scales, but with some loss of...

27 March 2020 2,483 3 View

Transfection in HFL-1 cells?

I am trying to transfect HFL-1 cells with a plasmid DNA. I used Lipofectamine 3000 from ThermoScientific, however the cells seem to react quite sensitive to the transfection (tried different...

17 November 2019 484 2 View

Why gap opening is fixed in instruments when using cone-plate geometry?

Hello I work with starch gels and I am performing experiments using cone and plate geometry with 1mm of gap because that height allows me to handle the samples better. I still can not understand...

28 March 2019 1,532 8 View

Does Golgi-cox stanning work in tissues storaged in sucrose 30% for 1 month before the stainning?

11 December 2018 5,386 1 View

Transforming a Count Variable for use in Regression?

I am currently working on my dissertation and am trying to convert 3 separate count variables/multiple response variables for use in multiple regression. I am using SPSS to analyze the data. Any...

23 November 2018 5,738 10 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to threshold an image despite bright lighting, using ImageJ/Fiji?

Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...

27 February 2021 1,194 3 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View