Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Has anybody seen research on Generative Adversarial Networks not image / video / autoencoder based?

Searching for Generative Adversarial Networks applications.

09 October 2020 2,316 2 View

Can the crystallinity of starch or other materials be calculated from the birefringent area?

Hi I would like to know if is it possible to obtain the crystallinity of a material such as starch from the ratio of the birefringent area to total area using the images obtained from light and...

30 March 2020 952 5 View

Why gap opening is fixed in instruments when using cone-plate geometry?

Hello I work with starch gels and I am performing experiments using cone and plate geometry with 1mm of gap because that height allows me to handle the samples better. I still can not understand...

28 March 2019 1,532 8 View

Does Golgi-cox stanning work in tissues storaged in sucrose 30% for 1 month before the stainning?

11 December 2018 5,386 1 View

If I want to do PCR of Cryptosporidium and Giardia to test, is it better to use a nested one?

05 June 2017 2,654 7 View

Can anybody provide some possible methods to dispose heavy metal contaminated soil, specifically Cd contaminated soil for Cocoa Cultivation?

Can anybody provide some possible methods to dispose heavy metal contaminated soil, specifically Cd contaminated soil for Cocoa Cultivation? superficial soil layers (30cm) large land...

02 March 2017 5,722 9 View

What is a good C-ter FLAG-tagged mammalian expression vector?

I need to subclone my GOI in a mammalian expression vector with a C-ter FLAG tag (or a dual FLAG-HA tag) for IP and affinity purification. My protein cannot properly fold with an N-ter tag, hence...

23 October 2016 146 1 View

Where is the problem with bad results on miRNA RT-qPCR?

In our lab we are performing an miRNA isolation from rat liver with Trizol (including the extra step for helping in the recovery of miRNAs) and then we perform the RT reaction with Taqman microRNA...

11 April 2016 3,121 8 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

01 March 2021 3,905 5 View