How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to smooth the energy production peaks of PV and wind energy?

Is power-to-gas a possible solution to this issue? Storing renewable energy in the form of H2 and CH4 and using them as fuels can increase the penetration of renewable energy in the transportation...

28 October 2020 3,490 3 View

Which are the most promising kinds of feedstock for renewable fuels?

Which kinds of biomass, byproducts and waste are the most interesting for new research development in anaerobic digestion and gasification?

03 May 2020 1,434 25 View

Power-to-gas from renewable energy sources: pros and cons?

Based on your expertise, what are advantages and disadvantages of producing H2 or CH4 from renewable sources (wind, PV etc)? I am mainly interested in collecting different points of view, e.g....

28 April 2020 1,926 8 View

Where do I find the Wilcox H test?

Hi all, I need to compare some population means, but my data are non-normally distributed and with unequal variance (even after transformation) so I can use neither parametric t-tests nor...

10 February 2020 6,485 7 View

Biohydrogen and dark fermentation: what is their potential?

Is the conversion rate a limiting factor of this technology, compared to gasification and hydrogenotrophic/acetoclastic methanogenesis? And does hydrogen have the chance to become a successful...

06 January 2020 3,349 5 View

Hoe can you cut hislogical section cuntaining calcium fragments??

Hello everybody, I have to cut histological sections cuntaining calcium fragments. Obviously the section are perforated at the bone fragments and I can't recover row sections. Do you have any...

30 July 2019 4,829 1 View

Is there a standard procedure to pre-process continuous blood pressure data?

Is there any standard Matlab pipeline/toolbox to preprocess continuous blood pressure (BP) data? I have collected BP and ECG data simultaneously and would like to know which filters I should use,...

14 March 2019 4,888 3 View

Which is the best SSR primer set for varietal identification of T. monococcum and of T. aestivum? Are there any reference databases?

I'm looking for a panel of DNA primers for performing varietal identification of T. monococcum and T. aestivum. In addition I'm looking for a reference database of varieties and microsatellite values.

04 February 2019 5,053 2 View

Could someone help me in the identification of this barnacle (Crustacea, Cirripedia)?

02 November 2016 3,927 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View