Valerio Piscopo

4 Questions 9 Answers 0 Followers

Questions related from Valerio Piscopo

Is there an exisiting protocol to differentiate iPSC into ependymal cells?

Hi everyone, I'm trying to understand if anyone has ever generated ependymal cells from iPSC, apparently there is not much in literature on the topic and I don't think is a trivial matter. Any...

25 January 2021 5,356 1 View

What is a good antibody to check NFkB pathway activation?

I'm studying the role of NFkB in gliogenesis and I need a good working antibody to check NFkB activation downstream of IKK proteins. I thought about p65, Rel A and so on. Regarding p65 there are...

11 September 2014 3,080 3 View

bHLH factor and chromatin remodeling in neural development. Any evidence?

I want to assess if a bHLH transcription factor (Ascl1) is able to open chromatin (e.g. by interaction with repressor complexes ot HDAC) of a specific target gene to promote the access of another...

11 May 2013 7,041 3 View

What is the best method to over-express a gene in neurospheres or neurosphere-derived cells?

I usually use lentiviral vectors but there's some problems with toxicity/efficiency... Could you share your experience?

10 May 2013 2,361 4 View