I want to know the basic information about the 16s rRNA, and want to use this technique in meat microbes identification. Can any body sent me related research articles especially from journal of meat science and food chemistry??
Thanks
Hi Zubair,
16 rRNA is considered as a fingerprint gene. 16S ribosomal RNA is the component of the 30S small subunit of a prokaryotic ribosome that binds to the Shine-Dalgarno sequence. It is considered as conserved region in the genome. based on this you can use for identification of your sample by sequencing of this gene and constructing phylogenies. you can see many articles relevant to this, e.g. see the below links.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC523561/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2772595/
https://www.nature.com/articles/srep32165
you can find more information and articles related to this.
Regards
Thanks for your precious time and comments Ghaffar bro., I will read it carefully and try to learn it. I immensely thanks from the core of my heart.
16S r RNA gene within bacterial DNA.This gene expresses the necessary protein for strucure and consistency of bacterial ribosome.This bacterial ribosome consists of rRNA and protein formed after translation of mRNA carrying the genetic code transcribed from 16S rRNA gene.
This 16S rRNA gene is a marker and fingerprint for bacterial identification i.e. its sequence differs among bacteria.So , you can use its specific primer and the amplified PCR product can be electrophoresed via agarose gel and can be cut from agarose and sequenced easily.Comparison of this sequence of 16S rRNA gene with that stored in gene bank using Basic Local Search Tool Programme (BLAST Programme) can show you the similarity between this sequence and that of gene bank . This BLAST give you both pairwise analysis and cluster analysis(Dendrograme).If the similarity is 99% ,this means that your isolate is named as the name of the neighbour and similar isolate.
A lot of thanks Dr. Enan for such a useful comments on the 16s rRNA.
Ribosomal ribonucleic acid (rRNA) is the RNA part of the ribosome and is essential for protein synthesis in all living organisms. rRNA is one of the gene products present in all cells. (16S rRNA is highly conserved sequences in prokaryotes, which is approximately 1,500 bp long and contains nine variable regions interspersed between conserved regions. Studies regarding estimate of the microbial communities using NGS thus far, have relied upon targeted sequencing of the hyper variable regions of SSU rRNA gene (e.g., V1, V3, V4, V6 regions)
https://www.nature.com/articles/srep32165
Dear Ahmad bro.,
Thanks a lot for sharing such a useful information about 16s rRNA.
Dear. I think the follow article can help
Article Direct 16S rRNA Gene Sequencing from Clinical Specimens, wit...
Greeting
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Microorganisms