I am plan on performing IHC using free floating coronal brain sections for c-FOS protein using cell signaling primary antibody 1:5000 for O/N incubation. This IHC worked very well for me when I did DAB staining for FOS. But I am now planning on performing immunofluorescence staining using Biotin-SP (long spacer) AffiniPure Goat Anti-Rabbit IgG (H+L) (Code: 111-065-003, Jackson labs) and DyLight™ 405 Streptavidin (Code: 016-470-084, Jackson labs). Do you have any suggestions for the incubation times I should use for the biotin labeled secondary antibody and the Dylight 408 streptavidin? Thanks