Rashmi Dubey

3 Questions 5 Answers 0 Followers

Questions related from Rashmi Dubey

Is it possible for Cre positive mice to be negative for flox reporter gene (eGFP)?

I was genotyping some mice tail snips. It is a Cre Lox reporter mouse. The Cre is expressed using CRH promoter in the mouse brain and eGFP is the reporter gene. I isolated g-DNA and used it for...

13 January 2021 7,025 3 View

What incubation times do I use for immunofluorescence cFOS staining of brain sections?

I am plan on performing IHC using free floating coronal brain sections for c-FOS protein using cell signaling primary antibody 1:5000 for O/N incubation. This IHC worked very well for me when I...

01 December 2020 9,388 2 View

Does anyone know a good IHC protocol for FGFR1 detection on floating brain sections?

I am trying to standardize IHC for cFOS and FGFR1 detection on rat brain sections. I use 40 micron sections and have had good results for cFOS detection. But I have not had any positive results...

26 March 2020 675 5 View