What happens if we prohibit the cytokinesis but not the karyokinesis?

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Can I use DEAE Sepharose in place of DEAE Cellulose?

I have been trying to purify Amyloid Beta peptide using ion exchange chromatography. In the paper I am referring to, they have used DEAE Cellulose in their protocol. I have been using Q-Sepharose...

15 January 2023 2,501 0 View

How to reduce noise in OCP and EIS study using Potentiostat?

Dear all, I have been doing trials for accessing corrosion properties of steel rebar in chloride environment using Potentiostat, wherein my working electrode is steel rebar, reference electrode is...

15 November 2022 8,797 0 View

Dear all I wanted to Ask is there are any traditional method for isolation of T Cells?

is there any traditional method to check T cell levels or can we isolate T-cells to check Th17 cell levels using specific markers? I am a little stuck here. Just to clarify I don't want to use...

20 September 2022 1,266 1 View

Hi everyone. So I'm trying to locate nanoparticles inside plant tissues. Is there a technique I could use that can detect them without sectioning?

I need a rapid protocol for the detection of any spectral signature of nanoparticles inside plant tissues without necessarily sectioning the tissue. Is it possible?

28 August 2022 2,370 0 View

How to use Case study method in Design Management research?

I'm conducting research to understand the design management practices in early stage start-ups. I 'm taking a action research and case study method as my approach. Would like to connect with...

14 February 2022 7,613 0 View

What type of plasticity model and its data for wood material should be input in ABAQUS software?

I am an M.Tech structural engineering student working on the project ' Numerical analysis of Kath-kuni architecture ( a common masonry typology ) in Himachal Pradesh region of India subjected to...

11 January 2022 1,154 3 View

Is there a dataset available on actual individual person usage of OS? If not what is the best way to create it?

I want to create a N.N which can learn person OS usage based on their use of application and data related to specific application's background time, usage frequency, usage time of the application...

07 December 2020 1,611 4 View

If we have without any reference standard how are you calculate the activity of sample in gamma ray spectrometry?

Gamma ray spectrometry

09 April 2020 5,409 0 View

What is causing the delay in expression of EGFP in HEK293T transient transfection with pAAV plasmid?

I have an AAV2 backbone and I am trying to create viruses for my further experiments. I am making an AAV CMV EGFP virus as a negative control and it is giving me a problem. The EGFP expression in...

15 January 2020 4,205 1 View

Does anyone has any idea, how to get rid of TEA contamination in LC/MS?

We have Agilent 6300 series Ion trap in our lab. Last week I used TEA to reduce the tailing of the compound and after that a strong peak started to appear at 102 which is of TEA. Can anyone...

23 June 2019 4,072 10 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,589 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View