What happen to protein expression when we reduce the temperature and what happen when we introduce the heat shock stages to improves the solubility?

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Alemu Tekewe

The rate of protein synthesis will be reduced, and thus proteins will not form inclusion bodies.

Adam B Shapiro

Heat shock proteins act as chaperones to help refold incorrectly folded proteins and to aid newly synthesized proteins to fold correctly. Heat shock proteins can be induced by heat shock, or they can be co-expressed with the protein of interest.