6 Questions 5 Answers 0 Followers
Questions related from Shadariah Mamat
what happen to protein expression when we reduce the temperature and what happen when we introduce the heat shock stages to improves the solubility?
09 September 2017 2,107 2 View
hi there, My friend got a problem in preparing the PSM Media,anyone know how to prepare the media? currently she want try to grow the bacteria on the psm media at different temperature such...
11 November 2016 1,019 1 View
pET22b vector? any idea..elaborate more what i need to know about this vector?
11 November 2016 2,293 0 View
HI, can someone give me the method for competence cell preparation for BL21DE3 and DH5alpha, thank you.
09 September 2016 2,803 3 View
hi there, want to ask you what kind of vector that can be used to transform the clone into BL21DE3 for protein expression? Currently im using pET SYSTEM and seem its not working. So i decide to...
09 September 2016 9,275 2 View
Hi all, thank for give a comment, my problem is why i cant express my clone into DH5 alpha ? the expression seem to unsuccessful because there is no growing on plate. i have read an articles said...
01 January 1970 5,339 7 View
