pET22b vector? any idea..elaborate more what i need to know about this vector?
what happen to protein expression when we reduce the temperature and what happen when we introduce the heat shock stages to improves the solubility?
08 September 2017 2,123 2 View
hi there, My friend got a problem in preparing the PSM Media,anyone know how to prepare the media? currently she want try to grow the bacteria on the psm media at different temperature such...
10 November 2016 1,037 1 View
HI, can someone give me the method for competence cell preparation for BL21DE3 and DH5alpha, thank you.
08 September 2016 2,819 3 View
hi there, want to ask you what kind of vector that can be used to transform the clone into BL21DE3 for protein expression? Currently im using pET SYSTEM and seem its not working. So i decide to...
08 September 2016 9,294 2 View
Hi all, thank for give a comment, my problem is why i cant express my clone into DH5 alpha ? the expression seem to unsuccessful because there is no growing on plate. i have read an articles said...
01 January 1970 5,359 7 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
02 July 2024 5,448 2 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...
25 June 2024 1,197 0 View
I am working on a project that involves analyzing remote sensing (RS) images. I need to convert these images into vector data for further analysis. I am looking for automated methods or software...
22 June 2024 6,165 2 View
In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...
19 June 2024 7,822 3 View
Should we use special binary vectors when using plasmid rescue method to detect transgene integration site in plant? that is, there is often no ori site between LB and RB region of simple binary...
28 May 2024 1,873 0 View