How can one achieve preferential expression of a particular mRNA molecule transfected into a microbial species ?

In other words, how do you design a self-amplifying (replicon) RNA aiming to achieve the preferential expression of a particular mRNA? What other mRNAs need to be included in the replicon? Thanks.

08 September 2018 778 4 View

How likely is it that a piece of self-amplifying viral replicon RNA may be serendipatously taken up by cultured staphylococci and expressed in vitro?

The question adresses the possible transfection of bacteria with replicon RNA vaccine material to create a fagocytable microbial form susceptible of being used as vaccinating material.

08 September 2018 8,981 2 View

What design strategies are advisable for a drug needing to cross the blood-brain barrier but not enter brain cells?

Hopefully the question is self-explanatory. Answers will be much appreciated. Thanks.

03 April 2018 909 4 View

What interesting chemical peculiarities stand out in this peptide primary sequence with a view to designing a specific "blocking drug" ?

The problem molecule is a heme peptide. Your answers will be greatly appreciated.

03 April 2018 4,453 6 View

Seeking for a specialist in neurophysiology of learning for a joint article-writing project on psychotherapy and sleep, anyone interested ?

02 March 2015 2,405 6 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How to find a correlation between coordinates in a curvilinear coordinate system?

Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...

28 February 2021 364 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View