How can one achieve preferential expression of a particular mRNA molecule transfected into a microbial species ?

In other words, how do you design a self-amplifying (replicon) RNA aiming to achieve the preferential expression of a particular mRNA? What other mRNAs need to be included in the replicon? Thanks.

08 September 2018 778 4 View

How likely is it that a piece of self-amplifying viral replicon RNA may be serendipatously taken up by cultured staphylococci and expressed in vitro?

The question adresses the possible transfection of bacteria with replicon RNA vaccine material to create a fagocytable microbial form susceptible of being used as vaccinating material.

08 September 2018 8,981 2 View

What design strategies are advisable for a drug needing to cross the blood-brain barrier but not enter brain cells?

Hopefully the question is self-explanatory. Answers will be much appreciated. Thanks.

03 April 2018 909 4 View

What interesting chemical peculiarities stand out in this peptide primary sequence with a view to designing a specific "blocking drug" ?

The problem molecule is a heme peptide. Your answers will be greatly appreciated.

03 April 2018 4,453 6 View

No title

Thank you all

11 December 2016 7,699 0 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

An article on the Iranian state's civil liability in the COVID-19 matter needs supervision; Is anyone interested in collaborating?

Hello. An article on the Iranian state's civil liability in the COVID-19 issue has been written in collaboration with a friend and a comparative look at law and biology. The article is ready to be...

15 February 2021 2,906 2 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Why marketers need a forensic understanding of consumers and a creative, agile mindset?

02 February 2021 6,579 10 View

How can an Undergraduate collaborate with Professors globally for research projects?

I am seeking to increase my experience by working on research projects and aiming to present them as papers

01 February 2021 3,800 4 View

Are there well-established collaborative sorting tasks/datasets?

Hi, I am currently working on research on communication behavior in Mixed Reality. I am planning a study, where dyads perform a task, in which they have to collaboratively cluster or sort cards...

31 January 2021 4,979 3 View

Question cancelled Question cancelled ?

Question cancelled

19 January 2021 7,478 3 View

What are the most recently efficient and effective information security systems?

I am working on a project: The future of information security: system-approach. In this case I would like to invite you to collaborate in any way that suits you. Any valuable research project...

06 January 2021 5,514 6 View