I have been using 5-aza-2′-deoxycytidine (5-aza-dC) for DNMT inhibition in HEK293 cells, recently i have observed that the cells are not showing any response to the treatment. does the stability of this compound get effected after some time? i made the stock solution in PBS, should i be using DMSO instead?
Hi Mehrotra,
I don't remember the exact details, but I do remember that this reagent is extremely unstable once it has been dissolved (and it is also very unstable in the the cell medium; if you do several days of incubatios, it has to be replenished daily, or at least every other day). It is even recommended to dissolve it in cold PBS, and to freeze it or use it immediately. I think I prepared aliquots that I used only once (do not freeze thaw), and even those aliquots can be stored only for about a month at -20C. The powder on the other hand is quite stable at room temperature.
Good luck!
Hannah
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