How to design taqmanprobes and primers for methylation analysis on specific promoters and detect by real time PCR?

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Hello everyone, Do any of you have a suggestion for a good lysis buffer to be used on human CSF samples so that it can be further used for quanterix simoa assays/ immunoassays?

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What effects the stability of 5-aza-2′-deoxycytidine (5-aza-dC)?

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Geotechnical Engineering (Proceedings of the ICE) time review?

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How to confirm the site-directed mutagenesis result without performing NGS?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

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Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

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Cash Kumar

ok , thats nice to listen and i am happy to help. but are you also concern about HYDROXY methylation nad is it about MSP/BSP ?

Gautam Pandey

Try MethPrimer primer designing tool..All the best !!

Aanchal Mehrotra

I am trying to use Methylight PCR technology using 2 specific primers and a taqman probe, i think this Methprimer design tool just gives me primers for MSP and not taqman probe sequence.