Do any of you have a suggestion for a good lysis buffer to be used on human CSF samples so that it can be further used for quanterix simoa assays/ immunoassays?
I would dilute known 2~5X dilution of the fluid with PBS, 1ml aliquoted in 1.5ml E-tube, centrifuged at 12,000 rpm for 10 min to remove any possible particles, cell debris and aggregates to collect and pool the clear supernatant for further analysis along with similar processing with serum for monitor.
this tubes will be used for any analysis with known dilution factor.