Hi guys, I don't now the meaning of BT in the table. I've done some HiSeq projects but it's the first time to see BT. Any ideas?
Thanks
Dear all, I am interested in finding the core genome and singleton genes from a dataset of multiple chloroplast genomes (n=12). I don't have much experience in bidirectional BLAST. Can anyone...
06 July 2016 2,285 2 View
Dear all, One of my friends has to amplify some species-specific genes from cooking oil, which contained little and mostly degraded DNA. I think paleontologists often work on degraded ancient DNA....
06 July 2016 3,012 5 View
Can anyone send me the PDF? Thanks
04 May 2016 2,495 2 View
Hello, I know tef can be used as a phylogenetic marker for fungi. But what about other organisms? For eukaryotes, 18S rRNA is definitely too conservative in many cases. By the way, COI is used in...
01 February 2016 3,928 2 View
Hello, my data is from a functional DNA array and it looks like this : phylum SCC1 SCC2 SCC3 SDC1 SDC2 SDC3 SAC1 SAC2 SAC3 Verrucomicrobia 0.525962728 0.453958735 ...
11 December 2015 5,527 4 View
http://enveomics.blogspot.com/2012/11/semantics.html As showed above, this is a collection of handy scripts but haven't been deposited in github. I tried to find the dropbox download link but I...
07 August 2015 8,799 3 View
Hi everyone, I just started to use kmernorm to improve the single-cell assembly process. But after I finished, I didn't find any output (no new files or modified reads files). From the help menu,...
04 May 2015 9,260 0 View
Dear all, I don't have much experience of RNA-seq. I noticed that the reads number of one treatment (3 individuals) was lower than the other treatment. Similarly, the signal intensity of samples...
04 May 2015 3,343 9 View
Hi guys, I used laser-capture-microdissection (LCM) to pick the sponge-associated filamentous bacterial cells. Then I followed the instruction of QIAGEN repli-g single cell Kit to amplify the...
01 February 2015 2,535 11 View
Hi guys, I am doing a metatranscriptome study on marine sponges. In previous researches, the fungal rRNA genes and other fungal biogeochemical gene transcripts were detected by PCR and Geochip....
01 February 2015 6,162 5 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
AI-driven technologies offer previously unheard-of capabilities to process enormous volumes of data, extract insightful knowledge and improve predictive models, according to the UN’s World...
31 July 2024 9,733 3 View