Fabrizio Simeoni

4 Questions 5 Answers 0 Followers

Questions related from Fabrizio Simeoni

What does Triton X do in a ChIP seq sample?

I normally use this protocol for ChIPseq: https://www.nature.com/articles/nprot.2006.98 After chromatin sonication, it says to add 1/10 volume of Triton X 10% to the samples, spin to remove pellet...

09 July 2019 968 4 View

Issue with protein overexpression?

Hi, I am using a lentiviral vector to over express a protein in AML cells. I have cloned the flag-gene into the EIFalfa-MCS-SV40 vector. When I use this vector to simply transfect 293 cells, I can...

07 October 2016 978 3 View

ChIP-qPCR: same volume or same DNA concentration?

Hi, I am performing different ChIP-seq experiment but before sending the samples to sequence I want to check the enrichement with a qPCR. I checked using Nanodrop the DNA concentration of my IgG...

22 September 2016 4,281 4 View

Chip-seq. IgG and IPs have the same DNA concentration?

Hello, I'm doing a Chip-seq experiment and I've noticed that after DNA extraction I have the same amount of DNA in my negative control (IgG) and in the IPs? Do you think it's still worth it to...

28 February 2016 8,180 6 View