What does it mean by the absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm ?
absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm, does it mean the protein solution contain dimers/oligomers or does it contain nucleic acid contamination?
09 October 2015 9,906 3 View
Hello Different molecular weights proteins 23 kDa and 66 kDa looks similar band intensity on SDS-PAGE(coomasie staining) does it mean both proteins contain same concentration? When I measured...
09 October 2015 5,801 15 View
two proteins(23 kDa, 66 kDa) shown same band intensity on sds page with coomasie stainining. Then i measured the concentrations of both proteins with nano drop at 280nm directly, 1. with out...
09 October 2015 1,070 8 View
Hello
09 October 2015 4,103 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Dear all, I am working on analyzing data from a survey on student satisfaction. The survey contains items with a 7-point Likert response format that produce 12 scales related to different areas...
05 August 2024 2,141 4 View
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04 August 2024 5,974 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
Dear researchers, Why specific capacitance is not proportional to the specific surface area? I have material A with a surface area higher than material B, but material B is showing little bit...
26 July 2024 9,156 2 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.
20 July 2024 6,354 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View