Hello
What does it mean by the absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm ?
09 October 2015 9,150 0 View
absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm, does it mean the protein solution contain dimers/oligomers or does it contain nucleic acid contamination?
09 October 2015 9,906 3 View
Hello Different molecular weights proteins 23 kDa and 66 kDa looks similar band intensity on SDS-PAGE(coomasie staining) does it mean both proteins contain same concentration? When I measured...
09 October 2015 5,801 15 View
two proteins(23 kDa, 66 kDa) shown same band intensity on sds page with coomasie stainining. Then i measured the concentrations of both proteins with nano drop at 280nm directly, 1. with out...
09 October 2015 1,070 8 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
