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absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm, does it mean the protein solution contain dimers/oligomers or does it contain nucleic acid contamination?
10 October 2015 9,872 3 View
Hello Different molecular weights proteins 23 kDa and 66 kDa looks similar band intensity on SDS-PAGE(coomasie staining) does it mean both proteins contain same concentration? When I measured...
10 October 2015 5,777 15 View
two proteins(23 kDa, 66 kDa) shown same band intensity on sds page with coomasie stainining. Then i measured the concentrations of both proteins with nano drop at 280nm directly, 1. with out...
10 October 2015 1,050 8 View
What does it mean by the absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm ?
10 October 2015 9,135 0 View
Hello
10 October 2015 4,078 0 View