Greetings,
I am on a SeaHorse F24 assay with fibroblasts harbouring a mutation that affects mitochondrial functions. Most parameters (ATP levels, basal respiration, maximal respiration, spare respiratory capacity) are lower versus a healthy control which can be easily understood why. However, non-mitochondrial respiration is lower too. Could anyone tell me what it can mean or at least if it have sense?
Thank you!
Hello,
how do you measure non-mitochondrial respiration rates?
If you are using inhibitors (Rotenone, Antimycin A), you may be missing out on various pathways that are able to supply electrons without passing through CI, CII or CIII. A lot of recent studies discuss this, mainly on lower vertebrates and invertebrates but could still be a possibility. I mostly work on oroboros but the protocols are essentially the same.
Yes, it happened in my cells as well in similar type of mutations. It indicates that overall metabolism is suppressed or in other words the overall metabolism is slow down in these cells (may be because of energy stress). In my case growth rate of mutated cells were also down.
I agree with Rahul. While the bulk of oxygen is consumed by mitochondria in any cell, enzymatic reactions and oxygenases outside of mitochondria will also consume oxygen to a degree. If overall metabolism is decreased, one would expect non mitochondrial oxygen consumption to decrease as well.
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