What does 5-1 signify in tRNA nomenclature?

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How to solve the problem of unconscious teeth grinding during sleep at night?

One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...

08 August 2024 1,366 5 View

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to send my account link and how to make my account public?

Nothing

01 August 2024 755 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Should Coefficient of Variation (CV) in a biological data necessarily be below 30%?

In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...

27 July 2024 201 0 View

Why knockouts for same genes from two different gRNA are showing different result?

Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.

25 July 2024 8,313 1 View

How to interpret results of AST for Pseudomonas aeruginosa?

While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...

25 July 2024 9,229 1 View

Non-parametric version of the wo-way repeated measures ANOVA?

Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...

24 July 2024 3,284 6 View

Is possible to edit a gene present in a plasmid by Crispr Cas9?

Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...

22 July 2024 7,073 2 View

What is homomenthyl cinnamate?

the molecule name is citated in old literature about organic sunscreen molecules

10 July 2024 5,576 2 View

Query Regarding tRNA Annotation Order in Feature Table for NCBI Submission?

Dear Sir, I hope this message finds you well and in good health. I am writing to seek your guidance regarding an issue I encountered during the annotation of my phage sequence using DNA Master....

30 June 2024 8,650 0 View

What if different translations of the International Code of Zoological Nomenclature have different meanings?

Article 87 of the Code declares all translated versions of the Code to be "equivalent in force, meaning and authority." I'm curious about what happens if there is a difference in meaning between...

22 May 2024 2,911 3 View

How do we use exponents in the naming of polycyclic compounds?

I always encounter polycyclic compounds at work but I do not see many resources explaining how to name them. I know the basic of naming of bicyclic compounds (the ones placed inside square...

24 April 2024 1,575 3 View

Has anyone designed guideRNA for multiple targets using polycistronic-tRNA-gRNA expression system?

I am planning to design guideRNA for multiple targets to express as a polycistronic-tRNA-gRNA expression. I would like to know whether we need to include any linker between the tRNA and guideRNA...

29 March 2024 1,055 0 View

Autonyms in the International Code of Botanical Nomenclature?

Hola! I'm having some issues interpreting 22.1 and 22.2 articles. From what I've read if the type species of a subdivision of a genus is NOT "listed" in the autonym subdivision, then that makes...

22 January 2024 3,247 6 View

Sample Size Calculation formular - Leslie Kish or Leslie Fischer's?

Leslie Kish formular or Leslie Fischer's formular? What is the actual nomenclature? Are these names used interchangeably? The Literature isn't helping to clarify at all.

16 January 2024 6,394 3 View

What is your opinion on Article 60.3 of the International Code of Zoological Nomenclature?

Dear colleagues, According to the International Code of Zoological Nomenclature (Chapter 12, Article 60, especially 60.3 entitled "Junior homonyms without synonyms"), the substitutes for...

16 December 2023 6,279 5 View

What type of study (or studies) could prove or disprove the hypothesis of parthenocarpy in Cannabis sativa L.?

What type of study (or studies) could prove or disprove the hypothesis of parthenocarpy in the Cannabis L. plant genus?

09 October 2023 529 3 View

How to represent species complexes in binomial nomenclature?

What would be the correct way of stating that the particular taxon belongs to a species complex, during identification? For example, a gecko that belongs to the Hemidactylus brookii complex. Thank...

22 September 2023 942 6 View